TY - BOOK AU - Howe,C.J. TI - Gene cloning and manipulation SN - 9780521817936 (hardback) U1 - 572.86CHR 2007 PY - 2007/// CY - Cambridge, New York PB - Cambridge University Press KW - Cloning, Molecular KW - methods KW - Escherichia coli KW - genetics KW - Transformation, Genetic KW - Gene Library N1 - Includes bibliographical references (p. [250]-256) and index; The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms -- Examples ER -